The 1965 NCAA Men's University Division Ice Hockey Tournament was the 18th such tournament in NCAA history. It was held between March 18 and 20, 1965, and concluded with Michigan Tech defeating Boston College 8 to 2. All games were played at the Meehan Auditorium in Providence, Rhode Island. The ECAC tournament champion and the two WCHA tournament co-champions received automatic bids into the tournament. An at-large bid was offered to a second eastern team based upon both their ECAC tournament finish as well as their regular season record.